Real Experiments 3D
Datasets of the Challenge 2016
The page contains 2 real datasets (3D Astigmatism). They were published for the challenge 2016 and used to compare sotware packages.
Sage et al., Quantitative Evaluation of Software Packages for Single-Molecule Localization Microscopy Open-access PDF, Nature Methods 2019. Li et al., Real-time 3D Single-Molecule Localization ising Experimetal PointSpread Functions Open-access PDF, Nature Methods 2018.
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Tubulin-A647 3D Astigmatism
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NPC-A647 3D Astigmatism
Datasets and Beads Results of 8 Software
Real dataset Courtesy of Jonas Ries (EMBL)
- 3D astigmatic dSTORM image of nuclear pore complex
- Nup107-SNAP-BG-AF647 in U-2 OS cells, imaged using dSTORM with a cylindrical lens (150'000 frames).
- Calibration data for the dataset. Fluorescent beads adsorbed to a glass coverslide in water are stepped in Z at high SNR
Reconstruction CSpline
Whole FOV of Tubulin-A647 • 3D Astigmatism (30nm/pix)
Reconstruction CSpline
Whole FOV of NPC-A647 • 3D Astigmatism (20nm/pix)
