Super-Resolution Microscopy Hub

Results Tubulin-A647-3D

3D astigmatic dSTORM image of nuclear pore complex. Courtesy of Jonas Ries (EMBL)

Sage et al., Quantitative Evaluation of Software Packages for Single-Molecule Localization Microscopy Open-access PDF, Nature Methods 2019.
Li et al., Real-time 3D Single-Molecule Localization ising Experimetal PointSpread Functions Open-access PDF, Nature Methods 2018.

3D-DAOSTORM Temporal grouping: Yes Wobble correction: Yes (Check) Drift correction: Yes	MIAtool Temporal grouping: Yes Wobble correction: Yes (Check) Drift correction: Yes	Cspline Temporal grouping: Yes Wobble correction: Not required Drift correction: Yes	RapidSTORM Temporal grouping: No Wobble correction: Yes (Check) Drift correction: Yes
WaveTracer Temporal grouping: No Wobble correction: Not required Drift correction: Yes	ThunderSTORM Temporal grouping: Yes Wobble correction: Yes Drift correction: Yes	QuickPALM Temporal grouping: No Wobble correction: No Drift correction: Yes	SMAP-2018 Temporal grouping: Yes Wobble correction: Not required Drift correction: Yes

Convention reconstruction
Integrating all the frames

Tubulin-A647-3D • 2D

Real dataset Courtesy of Jonas Ries (EMBL)

3D astigmatic image of microtubules imaged using dSTORM with a cylindrical lens (112'683 frames)
Microtubules in U-2 OS cells, labeled with anti-alpha tubulin primary and Alexa Fluor 647-coupled secondary antibodies.
Calibration data for the tubulin dataset. Fluorescent beads adsorbed to a glass coverslide in water are stepped in Z at high SNR

Datasets Challenge 3D sample

Download Dataset Tubulin-A647-3D

File	Link to Download	Size
Tubulin-A647-3D-stacks_2.tif	Tubulin-A647-3D-stacks_2.tif	0 Mb
ThunderSTORM-Tubulin_30nmpix.tif	ThunderSTORM-Tubulin_30nmpix.tif	0 Mb
Tubulin-A647-3D-stacks_4.tif	Tubulin-A647-3D-stacks_4.tif	0 Mb
Tubulin-A647-3D-stacks_3.tif	Tubulin-A647-3D-stacks_3.tif	0 Mb
Tubulin-A647-3D-stacks_1.tif	Tubulin-A647-3D-stacks_1.tif	0 Mb
Tubulin-A647-3D-BEADS-as-sequence.zip	Tubulin-A647-3D-BEADS-as-sequence.zip	0 Mb
Tubulin-A647-3D-BEADS-as-stacks.tif	Tubulin-A647-3D-BEADS-as-stacks.tif	0 Mb