Dataset NPC-A647-3D • 3D
Real dataset Courtesy of Jonas Ries (EMBL)
- 3D astigmatic dSTORM image of nuclear pore complex
- Nup107-SNAP-BG-AF647 in U-2 OS cells, imaged using dSTORM with a cylindrical lens (150'000 frames).
- Calibration data for the dataset. Fluorescent beads adsorbed to a glass coverslide in water are stepped in Z at high SNR
Sage et al., Quantitative Evaluation of Software Packages for Single-Molecule Localization Microscopy Open-access PDF, Nature Methods 2019. Li et al., Real-time 3D Single-Molecule Localization ising Experimetal PointSpread Functions Open-access PDF, Nature Methods 15 2018.
Download Dataset NPC-A647-3D
| File | Link to Download | Size |
| ThunderSTORM-NPC_20nmpix.tif | ThunderSTORM-NPC_20nmpix.tif | 1 Mb |
| NPC-crop-10000-frames.tif | NPC-crop-10000-frames.tif | 286 Mb |
| NPC-A647-3D-BEADS-as-sequence.zip | NPC-A647-3D-BEADS-as-sequence.zip | 7 Mb |
| NPC-A647-3D-BEADS-as-stacks.tif | NPC-A647-3D-BEADS-as-stacks.tif | 22 Mb |
| NPC-A647-3D-stack_1.tif | NPC-A647-3D-stack_1.tif | 3641 Mb |
| NPC-A647-3D-stack_2.tif | NPC-A647-3D-stack_2.tif | 3642 Mb |