Super-Resolution Microscopy Hub
SRM HUB

SUPER-RESOLUTION MICROSCOPY HUB

Computational Resources for Super-Resolution Microscopy

Simulation MT0.N2.LD

feature,value 
Number of fluos,   8731
Number of frames,  20000
Field of view (nm), 6400.00
Number of activations,  24938
Average number of photons / activation, 497.696521
Density - number of fluorophores per frame, 1.246900
Density - number of photons per frame, 620.577792
Center of power illumination X (nm), 2948.519
Center of power illumination X (nm), 3473.742
Distance at half maximum illumination (nm), 3520.000
Distance at quarter maximum illumination (nm), 4480.000
Power illumination (W), 350.000
Framerate, 10.000
Maximum flux of photons per frame, 848.687
Threshold for activation (min photons),  8.487
Ton,  3.000
Tdark,  2.500
Tbleaching,  1.500

Parameters

Camera
Photon converter factor or Quantum efficiency (QE)0.90e-/Ph.
Resolution64pixels
Pixelsize100.00nm
Field of view6400.00nm
Optics
Wavelength660.00nm
Numerical aperture (NA)1.49
Autofluorescence
Background level (Gain)1.00
Background level (Poisson distribution)10.00
Camera Noise
Distribution: Gaussian (stdev)74.4
Distribution: Gamma300.0
Distribution: add poisson0.0020
Analog Digital Conversion
Electron conversion e- per ADU45.00DN/e-
Baseline100.00DN
Saturation65535.00DN
Quantization16-bit
Computational Parameters
Thickness1500.00nm
Frames (interval:1)From 1 to 20000
MultithreadingOff-1 Thread
Pixelsize to PSF convolution10.0nm
Pixelsize for autofluorescence100.0nm
Pixelsize of the camera100.0nm
File formatTIFF 16-bits

Excerpt

First Frame: 00001.tif

Random Frame: 07327.tif

Random Frame: 11562.tif

Last Frame: 19996.tif

Time projection

Average Intensity Projection

Camera Resolution: 100.0nm/pixel (Original size)

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High Resolution: 10.0nm/pixel (Enlarge this image)

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Maximum Intensity Projection

Camera Resolution: 100.0nm/pixel (Original size)

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High Resolution: 10.0nm/pixel (Enlarge this image)

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Report of simulation

FeatureCountMinMaxMeanStdev
ID249381.00024938.00012469.500455.883
X249382.0886398.7642935.322105.270
Y249383.1686398.5473018.019105.324
Z24938-667.568634.80857.1018.235
Frame249381.00019996.00010038.922366.656
Photons249388.5151143.131497.69719.059
Channel249380.0000.0000.0000.000
Frame ON249380.0000.0000.0000.000
Total249381.0001.0001.0000.032
Background Mean2493874.764361.847193.0116.260
Background Stdev249388.10892.03028.0690.970
Signal Mean2493857.760420.720192.4146.197
Signal Stdev249381.25773.78315.8130.561
Signal Peak24938168.000741.000298.1689.694
Sigma X249380.0000.0000.0000.000
Sigma Y249380.0000.0000.0000.000
Sigma Z249380.0000.0000.0000.000
Uncertainty249380.0000.0000.0000.000
Closest ID249380.00024928.000893.584121.896
Closest Distance249387.560442.953429.40513.726
Closest Count249381.0003.0001.0280.033
CNR24938-0.35825.1624.0420.140
SNR24938-0.34815.1183.3580.115
PSNR249382.65240.02412.1730.408
Unknown249380.0000.0000.0000.000