Super-Resolution Microscopy Hub
SRM HUB

SUPER-RESOLUTION MICROSCOPY HUB

Computational Resources for Super-Resolution Microscopy

Simulation MT0.N1.LD

feature,value 
Number of fluos,   8731
Number of frames,  20000
Field of view (nm), 6400.00
Number of activations,  24938
Average number of photons / activation, 4604.954740
Density - number of fluorophores per frame, 1.246900
Density - number of photons per frame, 5741.918065
Center of power illumination X (nm), 2948.519
Center of power illumination X (nm), 3473.742
Distance at half maximum illumination (nm), 3520.000
Distance at quarter maximum illumination (nm), 4480.000
Power illumination (W), 400.000
Framerate, 50.000
Maximum flux of photons per frame, 7852.507
Threshold for activation (min photons), 78.525
Ton,  3.000
Tdark,  2.500
Tbleaching,  1.500

Excerpt

First Frame: 00001.tif

Random Frame: 07932.tif

Random Frame: 00069.tif

Last Frame: 19996.tif

Time projection

Average Intensity Projection

Camera Resolution: 100.0nm/pixel (Original size)

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High Resolution: 10.0nm/pixel (Enlarge this image)

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Maximum Intensity Projection

Camera Resolution: 100.0nm/pixel (Original size)

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High Resolution: 10.0nm/pixel (Enlarge this image)

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Report of simulation

FeatureCountMinMaxMeanStdev
ID249381.00024938.00012469.500455.883
X249382.0886398.7642935.322105.270
Y249383.1686398.5473018.019105.324
Z24938-667.568634.80857.1018.235
Frame249381.00019996.00010038.922366.656
Photons2493878.78310576.8604604.955176.342
Channel249380.0000.0000.0000.000
Frame ON249380.0000.0000.0000.000
Total249381.0001.0001.0000.032
Background Mean24938324.6252532.7151053.86234.944
Background Stdev2493826.971770.128176.2346.403
Signal Mean24938267.7603301.0001019.93633.587
Signal Stdev249382.995526.10685.8073.250
Signal Peak24938758.0005599.0001603.54854.086
Sigma X249380.0000.0000.0000.000
Sigma Y249380.0000.0000.0000.000
Sigma Z249380.0000.0000.0000.000
Uncertainty249380.0000.0000.0000.000
Closest ID249380.00024928.000893.584121.896
Closest Distance249387.560442.953429.40513.726
Closest Count249381.0003.0001.0280.033
CNR24938-1.12226.7763.3670.120
SNR24938-1.08713.3042.8670.098
PSNR249382.06643.02911.2800.391
Unknown249380.0000.0000.0000.000